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Objective To observe NLS-KALA-SA-PTX (NKSP) for lung adenocarcinoma cell line A549 in vitro with paclitaxel monotherapy, and the mechanism thereof. Methods MTT assay was used to detect A549 cell proliferation influ-enced by different concentrations of NKSP (20, 40, 80, 100μg/L) and paclitaxel monotherapy (20, 40, 80, 100μg/L) for 24 h, 48 h and 72 h.. Subsequent experiments were divided into four groups, namely, group A (without any drug treatment), group B (added polypeptide 80μg/L of self-assembled nanoparticles, NKS), group C (80μg/L paclitaxel monotherapy) and group D (80μg/L NKSP). Flow cytometry was used to detect the cell apoptotic rates after 48 h and 72 h treatment in four groups. Western blot assay was used to analyse the protein expressions of bax and caspase-3 after 48 h and 72 h treatment in four groups. Results Both paclitaxel monotherapy and NKSP can inhibit the proliferation of A549 cells. The inhibitory rates of paclitaxel monotherapy group at 48 h and 72 h and NKSP group at 72 h showed an increasing trend in a dose-depen-dent manner (P<0.05). After treatment for 48 hours, the apoptotic rate was significantly higher in D group than that of C group (P<0.05). But the apoptotic rate at 72 h was lower in D group than that of C group (P<0.05). The protein expressions of bax and caspase-3 at 48 h were significantl lower in D group than those of C group, which were higher at 72 h in D group than those of C group (P<0.05). Conclusion Compared to paclitaxel monotherapy group, NKS promotes slow release of pa-clitaxol, which reduces the cytotoxicity and extends the antitumor effects.
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OBJECTIVE:To analysis the characteristics and regularity of the adverse drug reaction(ADR)occurred in our hospital. METHODS:307 ADR cases collected from 2004 to 2008 in our hospital were analyzed statistically in respect of patient’s age and sex,route of administration,categories of drugs,organs and systems involved in ADR and clinical manifestations,etc. RESULTS:Of total 307 ADR cases,more cases were woman;45.93% of cases were aged ≤18 a and 60 a. 80.13% of ADR cases were induced via intravenous administration. The incidence of ADR cases were mainly induced by antiinfective drugs,accounted for 48.53%. The most common manifestation of the ADR was lesion of skin and its appendants(27.97%),followed by injury of digestive system(16.10%). CONCLUSION:Great importance should be attached to rational use of anti-infective drugs and its management to reduce or avoid the occurrence of ADR and improve the quality of ADR reports.